E. coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology) (Methods in Molecular Biology) From Humana Press

Product Description

A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.
Product Details

* Amazon Sales Rank: #1911971 in Books
* Published on: 2003-07-03
* Number of items: 1
* Binding: Hardcover
* 328 pages

Editorial Reviews

Review
...a scientific review and a methodological "cook-book" of high quality. It is truly a highly practical handbook, useful for all scientists working with gene cloning and manipulation." - Folia Microbiologica

"...a very useful addition to laboratories investigating plasmids per se, and for those employing plasmids as vectors for gene cloning." - Microbiology Today

Book Info
Univ. of California, Berkeley. Offers step-by-step protocols for the manipulation of recombinant DNA. Discusses cutting-edge methods to manipulate plasmids and techniques to clone DNA into plasmid vectors and transform E. coli. Outline format. Halftone illustrations. For researchers.